Fig. 9.

Many retinal gap junctions are not stained with Cx36 antibody. (a,b) Labeling with antibodies to calbindin (blue), Cx36 (green), and calretinin (CR, red) show that Cx36 is intimately associated with AII amacrine cells and calbindin-stained bipolar cells in sublamina b (a), whereas in the outer plexiform layer (OPL) at the same location (b), the light Cx36 staining does not colocalize with calbindin staining of horizontal cells or an ON cone bipolar cell subtype (blue). c: The coupled matrix of S1/S2 cells is not localized with Cx36 immunoreactivity. d: Squares containing Cx36 immunoreactivity do not contain processes stained with anti-serotonin at levels above chance. e: The average density of Cx36 in the selected squares occurs in “holes” (blue patch in d) in the center of the S1/S2 signal. Scale bars = 20 μm in a,b, 12 μm in c.