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Effect of nhlF on NHase activity of the R. rhodochrous ATCC 12674 transformants. Each transformant was grown for 24 h at 28°C in MYP medium containing CoCl2 as indicated. The NHase activity was measured as described. Solid boxes, R. rhodochrous ATCC 12674/pLJK50; dotted boxes, R. rhodochrous ATCC 12674/pLJK60.
