Figure 1.

Uptake of Cd²⁺ into vacuolar membrane vesicles purified from DTY165 and DTY167 cells. Uptake of ¹⁰⁹Cd²⁺ by DTY165 membranes (A) or DTY167 membranes (B) was measured in the absence of MgATP plus (○) or minus GSH (1 mM) (□) or in the presence of MgATP (3 mM) plus (•) or minus (▪) GSH. ¹⁰⁹CdSO₄ and gramicidin-D were added at concentrations of 80 μM and 5 μM, respectively. (C) Rate of ¹⁰⁹Cd²⁺ uptake by DTY165 membranes plotted as a function of the total concentration of Cd²⁺ ([Cd²⁺]) added to uptake media containing 1 mM GSH, 3 mM MgATP, and 5 μM gramicidin-D. Values shown are means ± SE (n = 3–6).