Figure 2.

Purification of cadmium·glutathione complexes by gel-filtration (A) and anion-exchange chromatography (B and C). ¹⁰⁹CdSO₄ (20 mM) was incubated with 40 mM GSH at 45°C for 24 h, and the mixture was chromatographed on Sephadex G-15 to resolve a high molecular weight ¹⁰⁹Cd-labeled component (HMW-¹⁰⁹Cd·GS) from a low molecular weight component (LMW-¹⁰⁹Cd·GS) (A). The peaks corresponding to HMW-¹⁰⁹Cd·GS and LMW-¹⁰⁹Cd·GS were then chromatographed on Mono-Q and eluted with a linear NaCl gradient (- — -) (B and C). ¹⁰⁹Cd (cpm × 10⁻³) was determined on 5 μl aliquots of the column fractions by liquid scintillation counting. Solid bars denote fractions subjected to further manipulations.