Table 1.
Effects of different GSH-related compounds on uncoupler-insensitive 109Cd uptake by vacuolar membrane vesicles purified from DTY165 and DTY167 cells
| Compound |
109Cd uptake, nmol/mg/10 min
|
|||
|---|---|---|---|---|
| DTY165
|
DTY167
|
|||
| − MgATP | + MgATP | − MgATP | + MgATP | |
| Cd2+ | 5.8 ± 2.4 | 5.6 ± 1.5 | 4.3 ± 1.3 | 4.6 ± 2.1 |
| Cd2+ + GSH | 4.2 ± 1.2 | 37.4 ± 4.5 | 3.3 ± 1.1 | 8.3 ± 2.7 |
| Cd2+ + GSSG | 5.1 ± 3.2 | 3.8 ± 2.3 | ||
| Cd2+ + GS-CH3 | 4.5 ± 1.9 | 3.7 ± 3.1 | ||
| Cd2+ + Cys-Gly | 5.6 ± 3.2 | 6.9 ± 1.4 | ||
| Cd2+ + Cys | 7.0 ± 1.2 | 3.9 ± 1.0 | ||
| Cd2+ + Glu | 5.7 ± 1.1 | 5.2 ± 1.3 | ||
GSH, oxidized glutathione (GSSG), S-methylglutathione (GS-CH3), cysteinylglycine, cysteine, and glutamate were added at concentrations of 1 mM. MgATP, 109CdSO4, and gramicidin-D were added at concentrations of 3 mM, 80 μM, and 5 μM, respectively. Values shown are means ± SE (n = 3–6).