Table 3.
Effects of uncouplers and V-ATPase inhibitors on uptake of Cd·GS2 by vacuolar membrane vesicles purified from DTY165 and DTY167 cells
| Addition | Cd·GS2 uptake, nmol/mg/10 min
|
|
|---|---|---|
| DTY165 | DTY167 | |
| Control | 105.8 ± 12.4 (100) | 17.3 ± 2.7 (100) |
| Gramicidin-D | 77.8 ± 6.4 (73.5) | 9.8 ± 2.0 (56.6) |
| FCCP | 62.2 ± 11.4 (58.8) | 10.2 ± 1.6 (59.0) |
| NH4Cl | 89.8 ± 8.2 (84.8) | 10.0 ± 1.7 (57.8) |
| NH4Cl + gramicidin-D | 69.8 ± 12.0 (66.0) | 8.8 ± 2.2 (50.9) |
| Bafilomycin A1 | 81.8 ± 6.0 (76.6) | 12.8 ± 3.6 (74.0) |
| Bafilomycin A1 + gramicidin-D | 70.2 ± 12.2 (66.4) | 7.2 ± 2.4 (41.6) |
Uptake was measured in standard uptake medium containing 50 μM purified 109Cd·GS2. Bafilomycin A1, carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP), gramicidin-D, and NH4Cl were added at concentrations of 0.5 μM, 5 μM, 5 μM, and 1 mM, respectively. Values outside parentheses are means ± SE (n = 3–6); values inside parentheses are rates of uptake expressed as percentage of control.