Figure 4.

Efficacy of the three selected organic compounds on the development of EAE severity in SJL/J mice. Mice were challenged subcutaneously (100 μg in 0.15 ml PBS emulsified in an equal volume of complete Freund’s adjuvant) with the proteolipid epitope (p139-151) on days 0 and 7. Pertussis was administered 30 min after antigen injection on day 7 (0.25/ml containing 5 × 10⁹ inactivated organisms, i.v.). The organic compounds were injected i.v. on day 12 at a dosage of 50 μg in 0.25 ml 0.25% DMSO. The mean severity score for each experimental group was calculated to include those mice that did not exhibit any symptoms of EAE. For all groups n = 4, except for TJU103 (n = 3).