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. 1997 Jan 7;94(1):85–90. doi: 10.1073/pnas.94.1.85

Figure 4.

Figure 4

dTAFII230-(82–158) contributes to stable TBP binding. (A) Sequence alignment of N-terminal regions of dTAFII230 and yTAFII130. Dots indicate gaps introduced to maintain optimal alignment. Shading indicates residues identical or similar to each other. The positions of subdomains I and II are indicated. (B) TBP binding affinity of dTAFII230 N termini. GST–dTAFII230-(2–81) (40 pmol) was incubated with TBP (40 pmol) in the absence (lane 1) or in the presence of histidine-tagged dTAFII230-(1–81) (lanes 2 and 3) or histidine-tagged dTAFII230-(1–158) (lane 4 and 5) at 60 (lanes 2 and 4) and 200 (lanes 3 and 5) pmol. Binding experiments are represented as in Fig. 1C.