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Construction of skeletal/smooth chimeric MHC. Schematic drawing of skeletal/smooth chimeric MHC construct. A chimeric MHC cDNA was produced by ligating skeletal MHC cDNA containing the 5′ 2319 bp corresponding to the globular motor domain and a smooth MHC cDNA containing 993 bp corresponding to the C-terminal long α-helix domain of S1 and S2. ELC, essential light chain; RLC, regulatory light chain.
