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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Mol Immunol. 2007 Mar 7;44(11):2841–2849. doi: 10.1016/j.molimm.2007.01.035

Figure 2.

Figure 2

PKCδ regulates CIITA and MHC class II promoters but not IRF-1 promoter. (A) The effects of Rottlerin on the indicated promoter activity. RAW264.7 cells were transiently transfected with the luciferase reporter for 36 hrs. Cells then were divided into three, treated with none, IFN-γ with DMSO (DM), or IFN-γ with Rottlerin (RT) for 10hrs. (B) RAW264.7 cells were transfected with the indicated luciferase reporter along with the empty vector (EV), wild type (WT) or DN (DN) PKCδ. Two days after the transfection, cells were treated with IFN-γ for 12 hrs. Cell lysates were prepared to assess the luciferase activity. Luciferase activity in each group was normalized by β-galactosidase activity. The results were expressed as relative luciferase activity (RLA) against the value of the DMSO treatment or the empty vector transfection. Transfections were performed in duplicate and data are means ± SE of at least three independent experiments.