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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Mol Immunol. 2007 Mar 7;44(11):2841–2849. doi: 10.1016/j.molimm.2007.01.035

Figure 3.

Figure 3

PKCδ is necessary to recruit serine phosphorylated Stat1, CBP and p300 to the CIITA promoter to enhance histone acetylation. (A) Kinetics of Stat1 and IRF-1 recruitment to the CIITA promoter, and histone acetylation upon IFN-γ treatment. IFN-γ was added to RAW264.7 cells for the indicated time period. ChIP assays were performed with the antibody as indicated and PCR was carried out with the primers to detect the binding to CIITA pIV promoter. (B and D) RAW264.7 cells were treated with Rottlerin or DMSO for 1 hr prior to the treatment with IFN-γ for additional 2 hrs. (C and E ) RAW264.7 cells that stably expressing the empty vector (EV) or DN PKCδ (DN) were treated with IFN-γ for 2 hrs. ChIP assays were performed with the indicated antibody followed by PCR to measure the binding to the CIITA promoter. Input shows 2% of total cell lysates. Data are representative of at least three independent experiments.