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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Mol Immunol. 2007 Mar 7;44(11):2841–2849. doi: 10.1016/j.molimm.2007.01.035

Figure 5.

Figure 5

CIITA expression requires Stat1 that is phosphorylated at both tyrosine and serine residues. U3A cells were transiently transfected with the luciferase reporter driven by the CIITA (A) or IRF-1 promoter (B) together with wild type Stat1 (WT), Stat1(Y701F), and Stat1(S727A). EV indicates the empty vector. Two days after the transfection, human IFN-γ was added and cultured for additional 12hrs. Transfections were performed in duplicate and data are means ± SE of at least three independent experiments.