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. 2007 Jul 26;117(8):2337–2346. doi: 10.1172/JCI31909

Figure 2. Quantification of cholesterol levels in tissue, whole mouse, and plasma.

Figure 2

(A) Total liver and skin cholesterol levels of apoE–/– and LXRα–/–apoE–/– mice at 34 weeks of age. Data are expressed as mean ± SEM. n = 5 per group. P value is indicated. (B) Whole-body cholesterol levels of apoE–/– or LXRα–/–apoE–/– mice at 25 weeks of age. n = 4 per group. P value is indicated. (C) Total plasma cholesterol levels of apoE–/– or LXRα–/–apoE–/– mice at 14 and 34 weeks of age. Data are expressed as mean ± SEM. n = 9–15 per group. (D) Fast protein liquid chromatography (FPLC) analysis of plasma cholesterol content of apoE–/– or LXRα–/–apoE–/– mice at 14 weeks of age. Fasting plasma samples from 5 mice of each genotype were pooled for this analysis. (E) Expression of LXR target genes in peritoneal macrophages isolated from apoE–/– or LXRα–/–apoE–/– mice. DMSO (D), 22(R)-, 20α-, and 27-hydroxycholesterol were used at 3 μM, and GW3965 (GW) was used at 1 μM. PLTP, phospholipid transfer protein.