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. 1998 Mar 3;95(5):2050–2055. doi: 10.1073/pnas.95.5.2050

Figure 5.

Figure 5

Inhibition of GR transcriptional enhancement by JNK but not ERK is mediated through receptor phosphorylation of Ser-246. GR-deficient U-2 OS human osteosarcoma cells were transfected using the calcium phosphate precipitation method (28). Each 60-mm dish received 1.5 μg of pRK5-HA ERK with 4.0 μg of pRK5-HA f-sos or pRK5 plasmid (A) or 1.5 μg of pRK5-M2 JNK with 4.0 μg of pCMV5-racQ61L or pRK5 plasmid (B) as indicated. In addition, each dish received 0.7 μg of pCMV-GR (wt GR or GR S246A, as indicated), 1.25 μg of ΔGTCO-CAT, and 0.5 μg of pCMV-LacZ. Transfected cells were treated with 100 nM Dex for 8 h where indicated, harvested, and assayed for CAT and β-Gal activity. Each experiment was performed in duplicates, three times, with less than 5% variation.