Fig. 2.

Cosilencing of MPK1 and MPK2 attenuates expression of late systemin-induced wound response genes. 35S::prosys plants were infiltrated with pTRV-MPK1/2 and -GFP (Control). Five weeks later, transcript levels of Actin (internal control), the late genes PI-I and PI-II and the early genes AOS2, LoxD, and AOC were assessed by sqRT-PCR in leaf tissue. Ethidium bromide-stained agarose gels containing RT-PCR products are shown (colors inverted). The experiments are representative of 10 plants from three independent experiments for the late genes and of six plants from three independent experiments for the early genes.