Fig. 3.
Distribution of Sauria SINE subfamily EOC and subsubfamily EOC-I. (A) Genomic DNA from snakes, lizards and rodents‡ was amplified by PCR, using primers EOCconsF and EOCconsR (see Material and Methods). A discrete PCR product of ≈340 bp was generated in all three major lineages of advanced snakes (lanes 1–4 Viperidae; lane 5 Elapidae; lane 6, Colubridae) but not in other snakes (lane 7), lizards (lanes 8–10), or rodents (lane 11). (B) Phylogenetic relationships of snakes. (C) Genomic DNA from snakes, lizards, and rodents‡ was amplified by PCR, using Sauria SINE-specific subsubfamily primers EOC-I-F and EOC-I-R (see Material and Methods). A discrete PCR product of ≈315 bp was generated only in E. ocellatus (lane 1). Lane 1 E. ocellatus; lane 2, E. coloratus; lane 3, Macrovipera lebetina; lane 4, Crotalus horridus; lane 5, Notechis scutatus; lane 6, Natrix tesselata; lane 7, Boa constrictor; lane 8, V. griseus; lane 9, V. timorensis; lane 10, P. muralis; lane 11, T. kempi. M, size marker.