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. 2007 Jul 11;104(29):12111–12116. doi: 10.1073/pnas.0702969104

Fig. 1.

Fig. 1.

Incidence and spectrum of tumors in K14cre female mice carrying conditional Brca1F and p53F alleles. (A) Kaplan–Meier (K–M) survival curve of K14cre;p53F/F female mice (n = 32) showing a median tumor-free survival age (T50) of 288 days. (B) K–M curves of K14cre;p53F/F mice (blue curve) versus K14cre;Brca1F/F female mice (green curve; n = 11; T50 = 595 days; P < 0.0001) and K14cre;Brca1F/F;p53F/F female mice (orange curve; n = 50; T50 = 213 days; P < 0.0001). (C) K–M curve of K14cre;p53F/F mice (blue curve) versus K14cre;Brca1F/+;p53F/F female mice (pink curve; n = 19; T50 = 332 days; P = 0.218). (D) K–M curve of K14cre;Brca1F/F;p53F/+ female mice (red curve; n = 16; T50 = 407 days). Tumor types (mammary, skin, or other) are indicated for each female. Mice were killed when mammary tumors reached a diameter of ≈1 cm or skin tumors grew to a size of ≈0.7 cm. For mice with both skin and mammary tumors, the size of the latter was used as the criterion. (E) Southern blot analysis of tumor DNA to detect Cre-mediated deletion and spontaneous loss of Brca1 (EcoRV + StuI digest, Brca1 exon 14 probe) and p53 (BglII digest, p53 XbaI probe). Tumors (T) and control spleens (C) from the same animal are shown for representative female mice (1–6) of each genotype.