Fig. 3.

Both VP16 and VP22 are required for the accumulation of vhs protein in transiently transfected cells. HEK 293T cells were transiently transfected with the listed plasmids, harvested 48 h after transfection, solubilized as described in Materials and Methods, and analyzed by immunoblotting for the accumulation of vhs (upper blot), VP22 (middle blot), and VP16 (lower blot). Immunoreactivity to actin (arrow) is shown as a loading control. The results are representative of at least five independent transfection experiments.