Fig. 4.

Wild-type vhs protein is not degraded by the proteasome pathway in the absence of VP22. HEK 293T cells were transiently transfected with the listed plasmids. Six hours before harvesting, the cells were incubated with mock medium (lanes 1 and 11) or medium containing DMSO (lanes 2, 5, 8, and 12) or 10 μM MG132 (lanes 3, 6, and 9) or 15 μM lactacystin (lanes 4, 7, and 10). The cells were solubilized as described in Materials and Methods and analyzed by immunoblotting for the accumulation of vhs (top blot), VP16 (middle blot), and VP22 (bottom blot).