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VP22 and VP16 rescue the accumulation of a reporter gene coexpressed with vhs. HEK 293T cells were transiently transfected with the listed plasmids, harvested 48 h after transfection, solubilized as described in Materials and Methods, and analyzed by immunoblotting for the accumulation of (from top to bottom) vhs, EGFP, VP22, and VP16.
