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VP22 does not up-regulate the expression of vhs RNA. Cytoplasmic RNA was purified from HEK 293T cells harvested 48 h after transfection with the indicated plasmids. RNA (8 μg) was loaded onto denaturing formaldehyde gel, transferred onto a nylon membrane, and probed with 32P-labeled fragment containing the entire coding sequence of vhs. The ethidium-bromide-stained rRNAs served as loading controls.
