The CBC in frog eggs of different size. (A and B) Embryos of G. riobambae (3 mm in diameter). (A) The embryonic disk in an embryo stained for the cell borders. (B) Sagittal section from a st-13 gastrula stained for cell nuclei. The CBC is thick, and the archenteron is small. (C) Late gastrula of E. anthonyi (2 mm in diameter) stained for cell nuclei. (D) Size comparison between E. tricolor and D. auratus gastrulae. (E) Gastrula of E. tricolor (2 mm in diameter). (F) Gastrula of E. ingeri (3 mm in diameter). (G) Gastrula of D. auratus (egg, 3.5 mm in diameter). (H) Midgastrula of E. coloradorum (1.3 mm in diameter). (I) Late gastrula of E. coloradorum. (J) Midgastrula of E. coqui (3.5 mm in diameter). The drawings on the left give the outlines in sagittal view of G. riobambae, E. anthonyi, and X. laevis embryos. The embryonic disk, which includes the CBC, is shown in gray. a, archenteron; bl, blastocoel; cbc, circumblastoporal collar; d, disk; yp, yolk plug. [Scale bars: 250 μm (B, C, and G); 500 μm (A, E, F, and H–J); and 1 mm (D).]