The Lim1 immunostaining of X. laevis embryos. (A) Early gastrula. (B) Late gastrula. Lim1 is expressed in the notochord and in the anterior domain (arrows). (C) Neurula, focused on the pronephros anlage. (D) The same embryo shown in C, focused on the Lim1 expression in the CNS. b, blastopore; cns, central nervous system; dl, dorsal lip; n, notochord; p, pronephros.