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Structure of the switch helix in FhuA. (a) X-band EPR spectra of the R1 label incorporated along the H1 or switch helix in FhuA without (black line) and with (red line) substrate. (b and c) Crystal structure of FhuA showing the switch helix without (b) and with (c) substrate. In the crystal structure, the I28 side chain (highlighted in Corey–Pauling–Koltun rendering) is a surface-exposed helical site in the absence of substrate but is buried in the presence of substrate.
