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. 2007 Jul 5;104(29):11915–11920. doi: 10.1073/pnas.0704581104

Fig. 4.

Fig. 4.

In vitro comparison of ECs, SMCs, and MSCs in platelet adhesion assay. Cells were pretreated with heparinase II (in b, d, and f) or remained untreated (in a, c, and e) before platelet adhesion assay. Human platelets were plated onto culture surfaces seeded with MSCs (a and b), ECs (c and d), or SMCs (e and f). After 30 min, nonadherent platelets were washed away. Platelets were stained by using CD41 antibody (dark brown). (b) Arrow indicates an example of platelet staining. MSCs, ECs, and SMCs were visualized by H&E counterstaining. (Scale bar, 20 μm.) (g) The number of adherent platelets on MSCs, ECs, and SMCs after 30-min adhesion. The platelets from three donors were used in four experiments. MSC-1 and MSC-2 were from two different donors. ∗, P < 0.05, n = 4. (h) The effects of heparinase II treatment on platelet adhesion. ∗, statistically significant difference between the untreated and heparinase-treated samples (P < 0.05, n = 4).