Figure 5.

(A) Kinetics of GIF activation by IFN-γ. IFN-γ (1000 units/ml)-treated RAW cells nuclear extracts (15 μg) were analyzed in EMSA using GATE probe. (B) The same nuclear extracts were used with a labeled ISRE (from ISG-561) probe. Arrow shows the position of IRF-1 (ISGF-2). (C) Specific binding of GIFs to GATE. EMSA was carried out except that nuclear extracts were incubated with indicated molar excesses of various unlabeled oligonucleotides corresponding the IFN-response elements as competitors for 20 min before the addition of labeled GATE probe.