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. Author manuscript; available in PMC: 2007 Jul 17.
Published in final edited form as: J Immunol. 2005 May 15;174(10):5994–6004. doi: 10.4049/jimmunol.174.10.5994

Figure 5. Contribution of GM-CSF to TRICOM-mediated costimulation on antigen-specific T-cell precursor frequency and T-cell avidity.

Figure 5

Panels A–C: C57BL/6 mice were vaccinated with rV-LacZ/TRICOM (closed circles) or rV-LacZ/TRICOM in combination with rF-GM-CSF (open triangles). After 30 days, splenocytes were harvested. Panel A: β-gal–specific CD8+ T-cell precursor frequency as determined by tetramer staining. Inset number indicates percent β-gal tetramer+/CD8+ T cells of CD3+ T cells. Panel B: β-gal–specific CD8+ T-cell avidity as determined by tetramer dissociation. Inset panel depicts results that are normalized as the percentage of maximum tetramer binding. Panel C: β-gal–specific CD8+ T-cell avidity as determined by CTL assay. Inset panel depicts results that are normalized as the percentage of maximum lysis. Panels D–F: CEA-Tg mice were vaccinated with rV-CEA/TRICOM (closed circles) or rV-CEA/TRICOM in combination with rF-GM-CSF (open triangles). After 30 days, splenocytes were harvested. Panel D: CEA-specific CD8+ T-cell precursor frequency as determined by tetramer staining. Inset number indicates percent CEA tetramer+/CD8+ T cells of CD3+ T cells. Panel E: CEA-specific CD8+ T-cell avidity as determined by tetramer dissociation. Inset panel depicts results that are normalized as the percentage of maximum tetramer binding. Panel F: CEA-specific CD8+ T-cell avidity as determined by CTL assay. Inset panel depicts results that are normalized as the percentage of maximum lysis.