Table 1.

Enzymatic and stability properties of wild-type TLP-ste, thermolysin, and the 8-fold mutated variant

Protease	Enzymatic properties at 37°C				Stability
	Specific activity casein, units/μg	kcat/Km		Ki	Topt	Half-lifes
		FaGLa (M−1⋅s−1) ×10−3	FaAFa (M−1⋅s−1) ×10−3	Phosphoramidon, nM	Casein, °C	80°C (min.)	90°C (min.)	100°C (min.)
TLP-ste	3.5  ± 0.7	30  ± 8	222  ± 33	50  ± 18	74	17.5	1.5	<0.5
TLN	NA	NA	NA	NA	77	>200	12.5	1
Eightfold mutant	3.9  ± 0.7	30  ± 6	266  ± 23	43  ± 13	95	Stable	Stable	170

NA, not applicable.