Table 2.

Effect of denaturing agents on activity toward furylacryloylated peptides at 50°C

GndHCl, M	Relative activity		Urea, M	Relative activity		SDS, % wt/vol	Relative activity
	TLP-ste	Eightfold mutant		TLP-ste	Eightfold mutant		TLP-ste	Eightfold mutant
0	100	100	0	100	100	0	100	100
1.0	99	95	0.5	96	97	0.25	72	94
2.0	62	83	1.0	87	91	0.5	37	81
3.0	0	68	2.0	75	93	0.75	9.6	57
4.0	0	50	3.0	3	86	1.0	0	40
5.0	0	40	4.0	0	72	1.25	0	29
5.5	0	15	5.0	0	63	1.5	0	22
6.0	0	0	7.5	0	35	2.0	0	0

Activities are expressed as percentage of the activity in the absence of denaturant.