Table 3.
Stabilities of the 8-fold TLP-ste mutant and a selection of enzymes from extremophiles
| Enzyme | Source | Half-life, hr | T, °C | Ref. |
|---|---|---|---|---|
| 2-Ketoisovalerate-ferredoxin oxidoreductase | Thermococcus litoralis | 0.8 | 95 | (41) |
| Carbamoyl-phosphate synthetase | Pyrococcus abyssi | 3 | 95 | (42) |
| Sulfide dehydrogenase | Pyrococcus furiosus | 12 | 95 | (43) |
| α-Glucosidase | Thermococcus AN1 | 0.6 | 98 | (44) |
| 3-Phosphoglycerate kinase | Pyrococcus woesei | 0.45 | 100 | (45) |
| Glyceraldehyde-3-phosphate dehydrogenase | Pyrococcus woesei | 0.7 | 100 | (46) |
| DNA-RNA polymerase | Thermoproteus tenax | 2 | 100 | (26) |
| Hydrogenase | Pyrococcus furiosus | 2 | 100 | (47) |
| Glyceraldehyde-3-phosphate dehydrogenase | Thermotoga maritima | >2 | 100 | (48) |
| Eightfold TLP-ste mutant | B. stearothermophilus | 2.8 | 100 | (This study) |
| ADP-dependent glucokinase | Pyrococcus furiosus | 3.6 | 100 | (49) |
| Amylase | Pyrococcus woesei | 6 | 100 | (26) |
| Glutamate dehydrogenase | Pyrococcus furiosus | 10 | 100 | (50) |
| β-Glucosidase | Pyrococcus furiosus | 85 | 100 | (51) |
| Cellobiohydrolase | Thermotoga sp. | 1 | 108 | (26) |
| α-Amylase | Pyrococcus furiosus | 2 | 120 | (52) |