Table 1.

Sequences of the LxxLL peptides identified by phage display screening of the (x)₇LxxLL(x)₇ library using purified hVDR in the presence of VDR agonist or antagonist. Leucine residues are highlighted in gray. Boxes indicate residues N-terminal to the LxxLL motif that display high sequence similarity. Asterisks indicate LxxLL peptides confirmed to bind mammalian hVDR in the two-hybrid assay (see Fig 1). The sequence of the known LxxLL motif in the DRIP205 protein is included for sequence comparison.

DRIP205					N				L	M	N	L	L	K	D	N
Agonist	1	D	V	P	W	Y	G	G	L	T	E	L	L	G	V	L	E	S	R	E
	2		T		M				L	T	S	L	L	Q	N	G	V	D	H	V	*
	3		S		T				L	W	T	L	L	S	S	E	G	D	S	M	*
	4		Q		R				L	W	D	L	L	D	L	P	S	P	T	S	*
	5		G		S				L	M	Q	L	L	T	E	N	V	G	T	H	*
	6		D		Q				L	T	Q	L	L	R	S	Y	D	A	G	L	*
	7		S		M				L	V	D	L	L	E	L	Q	P	R	Y	L	*
	8		V		S				L	L	N	L	L	M	Q	D	L	L	D	G	*
Antagonist	9	D	A	P	T	S	L	S	L	Q	R	L	L	H	P	N	M	S	X	A
	10	D	A	V	P	P	P	S	L	S	D	L	L	T	R	Y	P	E	W	P
	11	D	A	P	W	G	R	S	L	N	S	L	L	D	G	Y	P	V	S	S
	12	H	A	A	E	L	A	N	L	P	R	L	L	A	S	Y	E	D	R	L
	13	L	D	L	E	G	S	S	L	N	V	L	L	R	P	K	I	D	G	M