Skip to main content
. 1998 Mar 3;95(5):2061–2066. doi: 10.1073/pnas.95.5.2061

Figure 3.

Figure 3

GST pulldown experiments demonstrating interaction between the finger domain of GATA-1 and the E1A-binding region (E1A-BD). (A) Mapping of the domain of CBP that interacts with GATA-1. Numbers indicate amino acids present in the in vitro translated CBP. (B) Mapping of the region of GATA-1 that interacts with CBP. Disr.Nf bears a deletion spanning amino acids 200–248. GST-GATA-1Δf lacks both fingers, GST-f(GATA-1) represents the finger region alone. GATA-1 binding to itself served as positive control. Twenty percent of in vitro translated product was analyzed directly (input).