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. 2007 Jul;18(7):2429–2440. doi: 10.1091/mbc.E07-01-0011

Figure 7.

Figure 7.

Role of Rsp5 WW domains in vivo. (A) Immunoprecipitations of triple HA-tagged Bsd2 with protein A fusions of Rsp5 WW domain mutants. The blot was probed with the monoclonal anti-HA antibody 12CA5. Eluates were enriched over extracts by a factor of 50. Rsp5 was visualized in the eluates by peroxidase-coupled anti-peroxidase, and the eluate was diluted by a factor of 50 for this. (B) Localization of GFP-Tre1 in wild-type cells (WT) or cells bearing rsp5 point mutations in WW2 or WW3. (C) Localization of GFP-tagged SmfΔN in Δrsp5 cells expressing Rsp5 with the WW1, 2, and 3 mutations. (D) Cadmium sensitivity of Δrsp5 cells carrying Rsp5 with either a deletion of the C2 domain, or mutations in WW domains 1, 2, or 3. (E) Localization of GFP-tagged SmfΔN in a Δtre1 Δtre2 strain complemented with a plasmid expressing either wild type Tre1 (Tre1WT) or a mutant in which the PPxY motif was changed to PPAG (Tre1PY). (F) Localization of GFP-tagged Bsd2 expressed in Δrsp5Δtul1 cells complemented by Rsp5 carrying mutations in single WW domains.