Figure 2.

RhoA and Rac1 involvement in the CNF1-mediated protection against apoptosis. (A and B) Graphs showing the percentages (±SD) of apoptosis in cells transfected with the constitutively active form of RhoA (A) or treated with the proteasome inhibitor lactacystin (B) before CNF1 exposure. In both cases, the CNF1-induced protecting effect against UVB-induced apoptosis is prevented. (C) Graph showing the percentages (±SD) of apoptosis in cells transfected with the siRNA against Rac1 and then exposed to CNF1. Silencing of Rac1, confirmed by Western blot (C, right), counteracts the CNF1-induced protection against UVB-induced apoptosis.