Figure 3.

CNF1 acts via the PI3K/Akt/IKK/NF-κB pathway. Immunoblots showing the amount of the activated form of Akt (pAkt) (A) or the amount of phosphorylated GSK-3α/β (B), a substrate of activated Akt, after CNF1 exposure (A.U., arbitrary units). (C and D) Graphs showing the percentage (±SD) of p65-positive nuclei in cells pretreated with wortmannin (C) or PGA1 (D) before CNF1 exposure. (E and F) Graphs showing the percentage (±SD) of apoptotic nuclei in cells pretreated with wortmannin (E) or PGA1 (F), before CNF1 exposure and UVB irradiation. Note that inhibition of the PI3K/Akt/IKK/NF-κB pathway counteracts the prosurvival activity of CNF1.