Figure 4.
CNF1 increases Bcl-2 transcription via the PI3K pathway. Graphs showing Bcl-2 (A) and Bcl-XL (B) mRNA levels after different times of CNF1 exposure in HEp-2 cells, normalized to GAPDH mRNA levels. The transcription of the Bcl-2 gene increases starting from 24 h of CNF1 treatment, whereas Bcl-XL gene transcription is not varied. (C) Graph showing Bcl-2 mRNA level after wortmannin exposure and CNF1 treatment. Note that blocking the PI3K pathway renders cells not responsive to the CNF1-induced Bcl-2 mRNA increase (AU, arbitrary units).