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poly(A)+ mRNAs colocalize with P-bodies. WT cells expressing a GFP-tagged version of Dcp2p were grown in glucose containing medium (Glu) (A), shifted for 10 min without glucose (10 min −Glu) (A and B). Poly(A)+ RNA was visualized by oligo(dT) hybridization and indirect immunofluorescence. (B) Colocalization of poly(A)+ mRNAs with Dcp2-GFP. Left, Dcp2-GFP. Middle, poly(A)+. Right, merge generated by Adobe Photoshop (Adobe Systems, Mountain View, CA).
