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Effect of translation initiation on Pab1p accumulation in P-bodies. (A) WT strain expressing a GFP-tagged version of Pab1p or Dcp2p grown in YPGlu (Glu) were exposed to 1 M KCl for 15 min. Cells were washed and resuspended in SC plus Glu supplemented or not with the same concentration of KCl and observed. (B) WT, cdc33–2 and prt1-63 strains expressing a GFP-tagged version of Pab1p or Dcp2p grown in SC medium plus glucose at 23°C were shifted to 39 or 37°C, respectively, for 1 h.
