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Brachyury and Shh expression in normal and TCTP-null embryos. Whole-mount in situ hybridization of wild-type and TCTP-null embryos at various stages as indicated was carried out using riboprobes specific to Brachyury (A and B) or Shh (C). (C) Left, E8.5 TCTP+/− embryo; right, E9.5 TCTP−/− embryo. am, axial mesendoderm; nt, notochord; n, node; ps, primitive streak; endo, endoderm. Scale bar in all panels, 100 μm.
