Figure 4.

Histological analysis of E5.5 control and TCTP-null embryos “genotyped” by immunostaining. Sagittal sections of E5.5 embryos derived from heterozygous intercrosses were first stained with TCTP-specific antibody and visualized by confocal microscopy as described in Materials and Methods. After immunostaining (right panels of B and D), the same section was stained with hematoxylin and eosin (HE; left panels of B and D). (A and C) HE staining of the most central 4-μm sections of the same embryos as that shown in B and D, respectively. TCTP-expressing N (A and B) and nonexpressing M littermates (C and D) are as indicated. ve, visceral endoderm; epi, epiblast.