Figure 6.

Increased apoptosis of TCTP-null mutants at the E6.5 but not E5.5 stage. Sections of TCTP(+) and TCTP(−) embryos at E5.5 (A and B) and E6.5 (C and D) stages were subjected to TUNEL analysis. Embryos were “genotyped” by immunostaining with TCTP-specific antibody. Apoptotic cells were stained brown. The graphs in E and F show mean percentages ± SEs of TUNEL-positive cells from three to four independent experiments. Only the difference between TCTP(+) and TCTP(−) embryos at the E6.5 stage was statistically significant; *p = 0.007; n = 3 (F). (D) An arrow points to one example of apoptotic cells. Scale bar, 25 μm (A and B) and 50 μm (C and D).