Figure 7.

Reduced expression of D- and E- but not B-type cyclins in TCTP^−/− embryos. (A) Protein extracts from E9.5 embryos with the indicated genotypes were analyzed by immunoblotting using antibodies specific to various proteins as indicated. Lane 1 was the extracts pooled from three embryos with mutant phenotype as shown in Figure 2D. Asterisk (*) indicates cyclin D3 cross-reacted with cyclin D2 antibody. (B) Quantitative RT-PCR was carried out using RNA isolated from E9.5 embryos with normal or abnormal morphology as described in Materials and Methods. Shown here is the relative mRNA level of various genes as indicated in abnormal versus normal embryos. The results (mean ± SD) were plotted from two independent experiments done in triplicate.