Figure 5.

Role of ROS in menadione-induced cell death. (A) Flow cytometric study of the production of ROS in wild-type and btn1-Δ cells stained with 50 μg/ml MitoTracker Red CM-H2XRos after 200-min treatment with 0.1 mM menadione in the presence and absence of 100 mM l-NAME. The data are presented in the form of representative frequency histograms displaying relative fluorescence (x-axis) against the number of analyzed events (y-axis). (B) Survival after 200-min treatment with 0.1 mM menadione of wild-type, btn1-Δ, sod1-Δ, sod1-Δ btn1-Δ cells in the presence or absence of 0.4 mg/ml l-arginine.