Figure 8.
Role of yeast metacaspase in menadione-induced cell death. (A) Measurement of intracellular caspase-like activity (or other protease able to cleave substrates after Asp residue; ASPase) by the use of D2R in wild-type and btn1-Δ cells after 200-min treatment with 0.1 mM menadione in the presence or absence of 100 mM l-NAME and 0.3 mg/ml l-arginine. The data are presented in the form of frequency histograms displaying relative fluorescence (x-axis) against the number of events analyzed (y-axis). (B) Viability of cells deleted in the metacaspase YCA1 after 200-min treatment with 0.1 mM menadione.