Figure 1.

AAK1L is highly expressed in brain and heart tissue. (A) A schematic representation of the domain structure for the long and short isoforms of human AAK1 indicating the N-terminal serine/threonine kinase domain (STK), the middle domain that is enriched in glutamine, proline, and alanine (QPA), and the two clathrin-binding motif containing domains (CBD1 and CBD2). The regions used to generate antisense RNA probes for Northern blot analysis are indicated as probes 1 and 2. The unique 3′ sequence of AAK1L and its predicted amino acid sequence are indicated below. Underlined amino acid residues indicate predicted clathrin-binding motifs (SLL, DLL, and LIDL). (B) Antisense probes 1 and 2 were used to probe two independent RNA tissue blots as indicated. Blots were also probed for actin to indicate polyA⁺ mRNA loading. Only one of the two control blots is shown.