Figure 6.

AAK1 colocalizes with the early endosome. tTA HeLa cells were fixed and stained for AAK1 (red) and early endosome antigen 1 (EEA1, green). AAK1 colocalizes (yellow, arrowheads) with EEA1 at small peripheral endosomes (A, and boxed regions magnified in A′ and A″) and perinuclear endosomes (C and D). AAK1 was visualized with polyclonal antisera raised against the N-terminal domain (rabbit 6370) and EEA1 was identified with affinity-purified, FTIC-conjugated antibodies (BD Biosciences). Scale bar, (A) 10 μm; (A′, A″, C, and D′) 5 μm.