Table 2.

G. metallireducens genes for which transcription was induced at least 10-fold with benzoate as the sole electron and carbon donor, relative to acetate

Gmet ORFa	Fold change	Gene name	Predicted function
Gmet_0108	12.9		ATPase
Gmet_0356	13.8		Conserved hypothetical protein
Gmet_0519	20		Permease
Gmet_0728	16.5		Conserved hypothetical protein
Gmet_1802	23.6		Aldehyde ferredoxin oxidoreductase
Gmet_1803	15.7		4Fe-4S binding
Gmet_2017	31.5		Hypothetical protein
Gmet_2057	31.9	ech^	Enoyl-CoA hydratase/isomerase
Gmet_2058	15.2	act*	Thiolase
Gmet_2059	16.4	adh	Short-chain dehydrogenase
Gmet_2064	13.2	tre^	Bacterial regulatory proteins, IclR
Gmet_2065	28.7	oxr	4Fe-4S binding
Gmet_2068	32.6	scsA*	Succinyl-CoA synthetase, alpha
Gmet_2071	14.1	ech*	Enoyl-CoA hydratase/isomerase
Gmet_2072	18.4	acd^	3-hydroxyacyl-CoA dehydrogenase
Gmet_2074	50.7	bamN^	Thiolase
Gmet_2075	19.1	bamM*	Acyl-CoA dehydrogenase
Gmet_2077	21.8	bamK	N-acetyltransferase
Gmet_2080	36.1	bamH^	NAD(P) diaphorase, HoxF
Gmet_2081	27.5	bamG	NAD(P) diaphorase, HoxU
Gmet_2083	27.3	bamF^	hydrogenase, delta subunit; HTH mo
Gmet_2084	20.6	bamE*	4Fe-4S binding, FAD binding
Gmet_2085	27	bamD^	4Fe-4S binding, cysteine rich
Gmet_2086	20	bamC*	4Fe-4S binding
Gmet_2087	36.4	bamB*	Aldehyde ferredoxin oxidoreductase
Gmet_2147	10.4	bamU^	Amidohydrolase
Gmet_2149	12.1	bamS	Hypothetical
Gmet_2150	33.4	bamR*	Dch
Gmet_2151	29.3	bamQ*	Had
Gmet_2152	28.9	bamP*	Electron transfer flavoprotein, alpha
Gmet_2153	10.2	bamO^	Electron transfer flavoprotein, beta
Gmet_2157	12		Response regulator receiver
Gmet_2260	22.4		Succinyl-CoA synthetase, alpha sub
Gmet_3305	27.2		Oah2

^a Genes in bold are located in the aromatics island of the genome.

* Genes encoding proteins with increased abundance during benzoate oxidation (Wischgoll et al., 2005).

^ Genes expressed during benzoate but not acetate oxidation (Wischgoll et al., 2005).