FIGURE 3.

Time course of cold-shock and non-cold-shock mRNA translation at low and high temperature. Time course of cspAmRNA (A) and cspDmRNA (B) translation carried out at 15°C (broken lines) and 37°C (continuous lines) in the presence of equivalent amounts of E. coli MRE600 S30 extracts prepared from cells harvested before (open symbols) and after 120 min cold shock (filled symbols) as described in Materials and Methods. Samples, withdrawn from each reaction mixture at the time intervals indicated for translation at 15°C (upper abscissa) and 37°C (lower abscissa), were subjected to hot TCA treatment to quantify the radioactive product synthesized. Further details are given in Materials and Methods.