FIGURE 1.

(A) Scheme for the native purification of any desired sequence (“RNA X”) using the new affinity tag. H₆ denotes a 6x histidine tag. (B) Sequence of the RNA expression vector pRAV23 with the relevant restriction sites and elements denoted. The asterisks above the AG step near the NgoMIV site denotes the cleavage site for the glmS ribozyme such that the adenine will be left at the 3′-end of the RNA product. The sequence between the EcoR1 and HindIII sites is inserted into pUC19 to yield pRAV23.