FIGURE 5.

Gene-silencing activity of siRNA B modified with wobble and/or s²U unit at central domain. SiRNA activity was tested in a dual fluorescence assay, as described in Materials and Methods. HeLa cells were transfected with siRNA duplexes in 5 nM concentration. A positive control: cells transfected with pDsRed2-N1 and pBACE–GFP plasmids only. Percentage of BACE–GFP protein expression was normalized to RFP protein level. Error bars correspond to standard deviations of results of at least three independent transfection experiments (±SD). siRNA symbols and abbreviations according to Table 2.